Purpose Breast conserving surgery (BCS) improves quality of life and self-perceived body image, and when paired with radiotherapy is comparable to mastectomy. Tumour to breast size ratio is a major determining factor when considering BCS. Scaffold-guided breast tissue engineering (SGTE) is a promising technique being studied to address large volume defects from breast surgery that can’t be compensated for with oncoplastic techniques. As manufacturing of personalised scaffolds can take up to 12 weeks, for malignant resection, ‘off the shelf’ options need to be available. This study aims to determine the common dimensions and volumes of breast cancer specimens in women undergoing BCS that would allow for the prefabrication of such scaffolds. Methodology Retrospective study of all surgical specimens of women who underwent BCS at the Royal Brisbane and Women’s Hospital between 01/01/2012 and 31/12/2022. 3-Dimensional measurements of the tumour and specimen were recorded allowing volumetric calculation. Resections for invasive ductal, invasive lobular and DCIS were included. Results 966 patients were included. The average volume of specimens was 57.24cm3. For specimens mediolateral was the largest axis (5.6cm) followed by superoinferior (4.1cm) and anteroposterior (3.9cm) which produced an ellipsoid shape. The density of breast parenchyma was 0.82g/cm3. Palpable WLEs produced the largest specimens compared to wire and ROLLIS. Invasive lobular specimens were largest according to histological subtype. Average tumour accounted for 5.91% of total specimen volume. Conclusion Results would suggest an ellipsoid shape for SGTE grafts and that women undergoing palpable WLE or for lobular cancers will have the largest defects to correct.