Purpose Needle stick injuries in veterinary are common, however, contracting zoonotic infections from such animal vaccine is extremely rare. We present a case of a patient with Salmonella soft tissue infection to a digit following self-inoculation with poultry Salmonella vaccine. Methodology This is a case study regarding a 19-year-old male who sustained an injury to his right ring finger with a poultry vaccine gun. This vaccine was an oil-based live attenuated formulation against Salmonella typhimurium. The patient presented with an entry site at the ulnar tip of his digit with swelling extending to his metacarpophalangeal joint. Symptoms continued to worsen and he underwent a series of operations with non-viable tissue debridement. Wound tissue cultures grew Salmonella and advice was sought from the Infectious Disease Unit. The patient improved following a course of targeted antibiotics. Results We present a rare case of a hand infection potentially caused by the constituent of an agricultural vaccine. Poultry Salmonella vaccine is usually composed of living attenuated Salmonella typhimurium within a freeze-dried pallet. Live attenuated vaccines administered to animals tend to carry 5 to 10 times greater concentration of bacteria when compared to human vaccination. Furthermore, significant local inflammation and tissue damage can be incited by the adjuvant products contained within the vaccines. Previous published series on hand trauma from poultry vaccination did not show zoonotic infections. Conclusion Contracting zoonotic infections from inoculation of animal vaccine is extremely rare. Resulting injuries are the combination of infection and the tissue damage caused by the adjuvant components that constitute the vaccine.