Biodegradable Temporising Matrix (BTM) has become a mainstay in the acute management of major burn injuries at the New Zealand National Burn Centre. Whilst tolerant to infection, there is a need to minimise infection developing within the open cells of the BTM during the integration phase. Topical antimicrobial solutions which have been used include 10% Povidone-Iodine, 0.015% Chlorhexidine Acetate with 0.15% Cetrimide, and 1% Acetic Acid. Although anecdotally these antimicrobial solutions have not adversely affected the ultimate performance of the BTM in the creation of a neodermis and graftable wound bed, we wanted to investigate the effects of these solutions on the structural integrity of BTM. In this study we report on the effects of these various antimicrobial solutions on the structural stability of the BTM dermal regenerative template.