Purpose: Bowel perfusion is a known risk factor for anastomotic leak. Indocyanine green (ICG) is commonly used in fluorescence angiography (FA) for real-time intraoperative perfusion assessment. Small and large bowel have different blood supplies, and small bowel with its rich vascular arcades is known to be more resilient to ischaemia compared to large bowel, though this is often not obvious on serosal inspection. Methodology: At the time of formation of ileocolic anastomosis, intravenous ICG 7.5mg was injected and fluoresence detected using near infrared camera. Perfusion was compared between small and large bowel limbs. Results: In a small case series of three consecutive patients who underwent ileocolic anastomosis with ICG assessment, there was a subjective and objective difference in fluoresence of colonic and ileal limbs, with differential percentages of 95% to 68%, 100% to 75% and 95% to 44%. All had macroscopically well perfused ileal and colonic limbs serosally at the time of anastomosis and there were no leaks. Conclusion: ICG is able to demonstrate visually the difference in small and large bowel perfusion. It is important to keep this in mind for any anastomoses, although further studies with more standardised measurements are needed.