Purpose: Evolutionary studies suggest a single-cell origin from Protista to complex eukaryotes. Enzymatic pathways, essential for the maintenance of cell integrity, appear to be preserved across phyla. Within nature, these basic processes occur between temperatures of 4°C to 40°C. This novel study investigated the feasibility of keeping an organ viable at equilibrium with environmental temperature; using an oxygenated perfusate with a balanced electrolyte composition similar to plasma; and whether this was associated with increased reperfusion injury. Methodology: 4 multi-visceral porcine abdominal blocks were perfused with an oxygen-enriched, balanced buffered electrolyte solution with albumin (4g/dl) and TPN (BaxterTM) at 25ml/kg. 10mg/dL of creatinine was added. Reperfusion was on a normothermic preservation rig as a surrogate to transplantation with whole blood (MAP = 60mmHg). Platelet-activating factor (PAF) levels were measured to assess the impact of 5 hours of isothermic preservation on reperfusion injury. This was compared to similar blocks of static cold-stored organs reperfused in the same manner. Results: PAF levels were not significantly increased on reperfusion. Histological examination suggested a mild degree of non-lethal injury. The organs appeared viable, with active aerobic respiration and ATP generation. Conclusion: The pilot study demonstrates that isothermic preservation is feasible and non-inferior to static cold storage over 5 hours. The ability to maintain organ viability at environmental temperature allows for the simplification of machine perfusion technology.