Purpose: Viability testing is a proposed advantage of normothermic perfusion. Rapid turnaround is central to be of clinical utility. Organs of the alimentary system have complex interplaying systems regulating function that cannot be tested with solitary perfusion. This study investigated, in a novel isothermic preservation model of composite porcine abdominal blocks, the scope of physiological testing for viability assessment. Methodology: 4 composite porcine abdominal organ blocks (liver, kidney, pancreas and small bowel) were retrieved from beating heart donors. These were perfused with an acellular oxygenated perfusate and compared to 4 blocks undergoing static cold storage. Arterial and venous glucose, ICG retention at 15 mins, insulin and GLP-1 in response to enteral glucose stimulation and creatinine clearance were evaluated. Serial ATP (liver, kidney) was assessed. Results: A reducing glucose gap with incrementing ATP levels indicated aerobic metabolism as opposed to gluconeogenesis in the liver and kidney. GLP-1 levels rose post enteral glucose stimulation followed by an insulin surge in keeping with normal physiological responses. The ICG retention of the perfused livers was <20% in all blocks and the kidneys demonstrated the ability to clear creatinine. Conclusions: The novel method was successful in displaying preservation of a composite porcine organ block and ability to test and maintain physiological process. ‘En bloc’ preservation may be useful for real time physiological testing and decreasing discard rates with extended criteria donors.